ABSTRACT
Context: Covid 19 pandemic which evolved in successive waves had profound pyschosocial impact on affected in-dividuals. Perceptions had impact on both individual and environmental level with potential behavioural conse-quences. The aim of the research is to study the psychosocial perception and psychological impact of COVID-19 among hospitalized COVID-19 patients. Methodology: The study was a mixed method research (Quan-Qual sequential design) conducted in the Covid wards of a tertiary care hospital in Coimbatore district. The psychological impact was assessed using the General Health Questionnaire, Perceived Stress Scale. The results of quantitative analyses and qualitative analyses were expressed as proportions and done using thematic analysis using grounded theory respectively. Results: About 55% of the hospitalized Covid-19 patients had psychological impact. On multivariate analysis, the factors which emerged as independent risk factors for presence of psychological morbidi-ties due to COVID were presence of high stress level, sleep disturbances and their perception of COVID as high threat. Conclusions: Focussed Counselling with specific reference to attend to spiritual health component in addition, would go a long way in diminishing immediate and long-term psychological impact due to covid-19 illness. © 2022, MedSci Publications. All rights reserved.
ABSTRACT
The E-learning system plays a vital role in the COVID-19 pandemic period, the study special focus on college students' perceptions towards E-learning system in the Puducherry Union Territory. The study chosen all the college students to collect perceptions towards E-learning through well-designed questionnaire over to Google forms, the 400 respondents are answered the questionnaire, the collected data were analyzed by SPSS and AMOS software's. The application of statistical techniques like one sample t-test, independed sample t-test and one-way ANOVA was used. The result shows the student are facing difficulties to attend class and understand the concepts of lessons. To know the E-learning application features are tough. It concludes students are facing struggle in the E-learning during the COVID-19 pandemic period. The study suggested to University, Government, Education institution, will take necessary steps to solve the drawbacks in the E-learning system during the COVID-19 pandemic period. Copyright © 2022, Anka Publishers. All rights reserved.
ABSTRACT
Dengue fever is a tropical disease spread worldwide, transmitted by the mosquito Aedes aegypti. It affects 100 million people worldwide every year and half a million cases of dengue hemorrhagic fever are registered. At present, it poses sever health burden as combined infections of COVID-19. Currently, as a combined infection with COVID-19, it is becoming a serious health burden. To identify the active molecule, Maestro V12.7 was used with different tools including LigPrep, Grid Generation, SiteMap, Glide XP Docking, Pharmachophores and MM-GBSA. The UNRESS tool was also used to assess the protein stability with this dengue protein. The docking result showed that all examined phytocomponents except berberine and -(+)-l-alliin had good docking scores of -8.577 (azadirachtin), -8.112 (curcumin), -7.348 (apigenin) and -6.028 (andrographolide). However, berberine and -(+)-l-alliin possessed good hydrogen-bonding interactions with RdRp. In addition, molecular dynamic simulations demonstrate that the complex of azadirachtin and dengue protein has a solid understanding of the precise interactions. As per the research results, the present research suggests that this is the first statement of azadirachtin against NS5 RNA-dependent RNA polymerase domain (RdRp), despite extensive research on this molecule in previous investigations. Furthermore, we anticipate that molecules such as curcumin, apigenin, and andrographolide would show beneficial effects while in vitro and in vivo studies are conducted on virally related objects. Since we performed ADMET and pharmacokinetic properties in this research, we feel that the phytochemicals of the screened anti-dengue molecules may not need to be evaluated for toxicological effects.
ABSTRACT
Dengue fever has become one of the deadliest infectious diseases and requires the development of effective antiviral therapies. It is caused by members of the Flaviviridae family, which also cause various infections in humans, including dengue fever, tick-borne encephalitis, West Nile fever, and yellow fever. In addition, since 2019, dengue-endemic regions have been grappling with the public health and socio-economic impact of the ongoing coronavirus disease 19. Co-infections of coronavirus and dengue fever cause serious health complications for people who also have difficulty managing them. To identify the potentials of mangiferin, a molecular docking with various dengue virus proteins was performed. In addition, to understand the gene interactions between human and dengue genes, Cytoscape was used in this research. The Kyoto Encyclopedia of Genes and Genomes software was used to find the paths of Flaviviridae. The Kyoto Encyclopedia of Genes and Genomes and the Reactome Pathway Library were used to understand the biochemical processes involved. The present results show that mangiferin shows efficient docking scores and that it has good binding affinities with all docked proteins. The exact biological functions of type I interferon, such as interferon-α and interferon-ß, were also shown in detail through the enrichment analysis of the signaling pathway. According to the docking results, it was concluded that mangiferin could be an effective drug against the complications of dengue virus 1, dengue virus 3, and non-structural protein 5. In addition, computational biological studies lead to the discovery of a new antiviral bioactive molecule and also to a deeper understanding of viral replication in the human body. Ultimately, the current research will be an important resource for those looking to use mangiferin as an anti-dengue drug. Supplementary Information: The online version contains supplementary material available at 10.1007/s43450-022-00258-6.
ABSTRACT
Dengue fever has become one of the deadliest infectious diseases and requires the development of effective antiviral therapies. It is caused by members of the Flaviviridae family, which also cause various infections in humans, including dengue fever, tick-borne encephalitis, West Nile fever, and yellow fever. In addition, since 2019, dengue-endemic regions have been grappling with the public health and socio-economic impact of the ongoing coronavirus disease 19. Co-infections of coronavirus and dengue fever cause serious health complications for people who also have difficulty managing them. To identify the potentials of mangiferin, a molecular docking with various dengue virus proteins was performed. In addition, to understand the gene interactions between human and dengue genes, Cytoscape was used in this research. The Kyoto Encyclopedia of Genes and Genomes software was used to find the paths of Flaviviridae. The Kyoto Encyclopedia of Genes and Genomes and the Reactome Pathway Library were used to understand the biochemical processes involved. The present results show that mangiferin shows efficient docking scores and that it has good binding affinities with all docked proteins. The exact biological functions of type I interferon, such as interferon-α and interferon-β, were also shown in detail through the enrichment analysis of the signaling pathway. According to the docking results, it was concluded that mangiferin could be an effective drug against the complications of dengue virus 1, dengue virus 3, and non-structural protein 5. In addition, computational biological studies lead to the discovery of a new antiviral bioactive molecule and also to a deeper understanding of viral replication in the human body. Ultimately, the current research will be an important resource for those looking to use mangiferin as an anti-dengue drug. Graphical Supplementary Information The online version contains supplementary material available at 10.1007/s43450-022-00258-6.
ABSTRACT
Dengue fever is a tropical endemic disease that is transmitted by the female Aedes aegypti mosquitoes around the world. In dengue patients, the severe vascular leak, bleeding and organ failure underlines the severity of this disease. It affects 100 million people each year and half a million cases of dengue hemorrhagic fever are recorded worldwide. In addition, the combined infections of COVID-19 and dengue fever cause serious health problems for those infected. Because polyphenolic compounds have the ability to regulate and restore the immune system, the intervention of biological tools is required to find an accurate polyphenolic compound from plants to combat these viral diseases. In this computational study, we used a range of software to explore the anti-dengue potential of the selected polyphenols, while the database was also used to explore diseased enzymes and drug targets in the dengue pathways of Homo sapiens. All examined polyphenols showed excellent docking values after molecular docking and also showed remarkable hydrogen bond interactions between the ligand and the dengue protein. Among the polyphenols tested, (R)-(+)-rosmarinic acid has a docking score of −8451 with an energy value of −59,860. After that, curcumin has the second best docking score of −8.221, followed by D - (+) - catechin (−7.042) and epicatechin (−7.069). In addition, molecular dynamic simulations demonstrate that the complex of (R) - (+) - rosmarinic acid and dengue protein has a solid understanding of the precise interactions. The current study suggests that this is the first report of such compounds against the dengue virus, despite extensive research on these substances in previous research. Moreover, we expect these polyphenols to show promising effects when used in antiviral research such as in-vitro and in-vivo studies. In addition, toxicological studies such as adsorption, distribution, metabolism, and excretion are required before further exploration of the polyphenols, as this will help to understand the biological processes of these potential polyphenols. © 2022 Elsevier Inc.